usp34 lentiviral activation particles (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology usp34 lentiviral activation particles

Deletion of <t>Usp34</t> results in age-related TMJ degeneration. (A) Representative images of Safranin O/Fast Green staining of Usp34 icKO mice at postanal (PN) 3 months (M) and 12 M. Scale bars: 100 μm for low and 50 μm for high magnification. (B and C) Quantitative analysis regarding cartilage thickness and modified Mankin score according to Safranin O/Fast Green staining. n = 6 per group. (D) Representative micro-CT images reveal the subchondral bone microstructure. Scale bars: 100 μm. (E) Quantitative analysis of subchondral bone parameters. (F and G) Representative images and quantitative analysis of western blot for MMP13, ADAMTS5, α-tubulin in ATDC5 cells transfected with Usp34 siRNA. (H) Relative mRNA expression of MMP3, MMP13, ADAMTS4, and ADAMTS5 in ATDC5 cells transfected with Usp34 siRNA.

Usp34 Lentiviral Activation Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tfiid/TFIID+Antibody/pmc12861080-84-8-14
Average 94 stars, based on 196 article reviews

usp34 lentiviral activation particles - by Bioz Stars, 2026-09

94/100 stars

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1) Product Images from "USP34 attenuates cartilage degradation in temporomandibular joint osteoarthritis by ANT1-mediated mitophagy"

Article Title: USP34 attenuates cartilage degradation in temporomandibular joint osteoarthritis by ANT1-mediated mitophagy

Journal: JBMR Plus

doi: 10.1093/jbmrpl/ziag004

Deletion of Usp34 results in age-related TMJ degeneration. (A) Representative images of Safranin O/Fast Green staining of Usp34 icKO mice at postanal (PN) 3 months (M) and 12 M. Scale bars: 100 μm for low and 50 μm for high magnification. (B and C) Quantitative analysis regarding cartilage thickness and modified Mankin score according to Safranin O/Fast Green staining. n = 6 per group. (D) Representative micro-CT images reveal the subchondral bone microstructure. Scale bars: 100 μm. (E) Quantitative analysis of subchondral bone parameters. (F and G) Representative images and quantitative analysis of western blot for MMP13, ADAMTS5, α-tubulin in ATDC5 cells transfected with Usp34 siRNA. (H) Relative mRNA expression of MMP3, MMP13, ADAMTS4, and ADAMTS5 in ATDC5 cells transfected with Usp34 siRNA.

Figure Legend Snippet: Deletion of Usp34 results in age-related TMJ degeneration. (A) Representative images of Safranin O/Fast Green staining of Usp34 icKO mice at postanal (PN) 3 months (M) and 12 M. Scale bars: 100 μm for low and 50 μm for high magnification. (B and C) Quantitative analysis regarding cartilage thickness and modified Mankin score according to Safranin O/Fast Green staining. n = 6 per group. (D) Representative micro-CT images reveal the subchondral bone microstructure. Scale bars: 100 μm. (E) Quantitative analysis of subchondral bone parameters. (F and G) Representative images and quantitative analysis of western blot for MMP13, ADAMTS5, α-tubulin in ATDC5 cells transfected with Usp34 siRNA. (H) Relative mRNA expression of MMP3, MMP13, ADAMTS4, and ADAMTS5 in ATDC5 cells transfected with Usp34 siRNA.

Techniques Used: Staining, Modification, Micro-CT, Western Blot, Transfection, Expressing

Deletion of Usp34 accelerates cartilage destruction during TMJ OA. (A) Representative images of Safranin O/Fast Green staining of SHAM and UBR-induced TMJ OA mice. Scale bars: 100 μm for low and 50 μm for high magnification. (B and C) Quantitative analysis regarding cartilage thickness and modified Mankin score according to Safranin O/Fast Green staining. n = 6 per group. (D) Representative micro-CT images reveal the subchondral bone microstructure. Scale bars: 100 μm. (E) Quantitative analysis of subchondral bone parameters. (F and G) Representative images and quantitative analysis of western blot for MMP13, ADAMTS5, α-tubulin in ATDC5 cells transfected with Usp34 siRNA following exposure to IL-1β. (H) Relative mRNA expression of MMP3, MMP13, ADAMTS4, and ADAMTS5 in ATDC5 cells transfected with Usp34 siRNA following exposure to IL-1β.

Figure Legend Snippet: Deletion of Usp34 accelerates cartilage destruction during TMJ OA. (A) Representative images of Safranin O/Fast Green staining of SHAM and UBR-induced TMJ OA mice. Scale bars: 100 μm for low and 50 μm for high magnification. (B and C) Quantitative analysis regarding cartilage thickness and modified Mankin score according to Safranin O/Fast Green staining. n = 6 per group. (D) Representative micro-CT images reveal the subchondral bone microstructure. Scale bars: 100 μm. (E) Quantitative analysis of subchondral bone parameters. (F and G) Representative images and quantitative analysis of western blot for MMP13, ADAMTS5, α-tubulin in ATDC5 cells transfected with Usp34 siRNA following exposure to IL-1β. (H) Relative mRNA expression of MMP3, MMP13, ADAMTS4, and ADAMTS5 in ATDC5 cells transfected with Usp34 siRNA following exposure to IL-1β.

Techniques Used: Staining, Modification, Micro-CT, Western Blot, Transfection, Expressing

USP34 deficiency suppresses mitophagy. (A) Representative images of immunohistochemical staining of LC3, Parkin, and PINK1 in the TMJ cartilages of SHAM and UBR-induced TMJ OA mice. Scale bars: 50 μm. (B and C) Representative images and quantitative analysis of western blot for LC3, Parkin, PINK1, and α-tubulin in ATDC5 cells transfected with Usp34 siRNA following exposure to CCCP. (D) Representative TEM images of ATDC5 cells transfected with Usp34 siRNA following exposure to CCCP. Subcellular structures with discernible mitochondria (yellow arrows) and bound by a double limiting membrane are identified as putative mitophagosome structures (red arrows). Scale bars: 200 nm. (E) ATDC5 cells stained with mitotracker and lysotracker transfected with Usp34 siRNA following exposure to CCCP. Scale bars: 20 μm. (F) ATDC5 cells stained with Mito-SOX transfected with Usp34 siRNA following exposure to CCCP. Scale bars: 20 μm.

Figure Legend Snippet: USP34 deficiency suppresses mitophagy. (A) Representative images of immunohistochemical staining of LC3, Parkin, and PINK1 in the TMJ cartilages of SHAM and UBR-induced TMJ OA mice. Scale bars: 50 μm. (B and C) Representative images and quantitative analysis of western blot for LC3, Parkin, PINK1, and α-tubulin in ATDC5 cells transfected with Usp34 siRNA following exposure to CCCP. (D) Representative TEM images of ATDC5 cells transfected with Usp34 siRNA following exposure to CCCP. Subcellular structures with discernible mitochondria (yellow arrows) and bound by a double limiting membrane are identified as putative mitophagosome structures (red arrows). Scale bars: 200 nm. (E) ATDC5 cells stained with mitotracker and lysotracker transfected with Usp34 siRNA following exposure to CCCP. Scale bars: 20 μm. (F) ATDC5 cells stained with Mito-SOX transfected with Usp34 siRNA following exposure to CCCP. Scale bars: 20 μm.

Techniques Used: Immunohistochemical staining, Staining, Western Blot, Transfection, Membrane

USP34 deubiquitinates and stabilizes ANT1. (A) Volcano plots showing the differentially expressed proteins of USP34-deficient cells from public proteomic dataset in the National Genomics Data Center under accession numbers: OMIX007639. (B) Heatmap showing the differentially expressed protein of USP34-deficient cells from public proteomic dataset (OMIX007639). (C and D) Representative images and quantitative analysis of western blot for ANT1 and α-tubulin in ATDC5 cells transfected with Usp34 siRNA following exposure to IL-1β. (E) Representative images of immunofluorescence staining of ANT1 in the TMJ cartilages of SHAM and UBR-induced TMJ OA mice. Scale bars: 50 μm. (F) Co-immunoprecipitation of USP34 with ectopically expressed ANT1 in HEK293T cells. (G) Immunoblot of ANT1-linked polyubiquitin. HEK293T cells were treated with 10 μM MG132 for 4 h after transfection with the indicated constructs. The cell lysates were subjected to immunoprecipitation with the indicated antibody. (H) Measurement of ANT1 degradation rate. HEK293T cells were transfected with the indicated constructs and treated with 10 mg/mL CHX.

Figure Legend Snippet: USP34 deubiquitinates and stabilizes ANT1. (A) Volcano plots showing the differentially expressed proteins of USP34-deficient cells from public proteomic dataset in the National Genomics Data Center under accession numbers: OMIX007639. (B) Heatmap showing the differentially expressed protein of USP34-deficient cells from public proteomic dataset (OMIX007639). (C and D) Representative images and quantitative analysis of western blot for ANT1 and α-tubulin in ATDC5 cells transfected with Usp34 siRNA following exposure to IL-1β. (E) Representative images of immunofluorescence staining of ANT1 in the TMJ cartilages of SHAM and UBR-induced TMJ OA mice. Scale bars: 50 μm. (F) Co-immunoprecipitation of USP34 with ectopically expressed ANT1 in HEK293T cells. (G) Immunoblot of ANT1-linked polyubiquitin. HEK293T cells were treated with 10 μM MG132 for 4 h after transfection with the indicated constructs. The cell lysates were subjected to immunoprecipitation with the indicated antibody. (H) Measurement of ANT1 degradation rate. HEK293T cells were transfected with the indicated constructs and treated with 10 mg/mL CHX.

Techniques Used: Western Blot, Transfection, Immunofluorescence, Staining, Immunoprecipitation, Construct

ANT1 overexpression rescues mitochondrial homeostasis in USP34-deficient cells. (A and B) Representative images and quantitative analysis of western blot for LC3, Parkin, PINK1, and α-tubulin in ATDC5 cells transfected with Usp34 siRNA or Lv-ANT1 , following exposure to IL-1β. (C) Representative TEM images of ATDC5 cells transfected with Usp34 siRNA or Lv-ANT1 . Subcellular structures with discernible mitochondria (yellow arrows) and bound by a double limiting membrane are identified as putative mitophagosome structures (red arrows). Scale bars: 200 nm. (D) ATDC5 cells stained with mitotracker and lysotracker after transfection with Usp34 siRNA or Lv-ANT1 . Scale bars: 20 μm. (E) ATDC5 cells stained with Mito-SOX after transfection with Usp34 siRNA or Lv-ANT1 . Scale bars: 20 μm.

Figure Legend Snippet: ANT1 overexpression rescues mitochondrial homeostasis in USP34-deficient cells. (A and B) Representative images and quantitative analysis of western blot for LC3, Parkin, PINK1, and α-tubulin in ATDC5 cells transfected with Usp34 siRNA or Lv-ANT1 , following exposure to IL-1β. (C) Representative TEM images of ATDC5 cells transfected with Usp34 siRNA or Lv-ANT1 . Subcellular structures with discernible mitochondria (yellow arrows) and bound by a double limiting membrane are identified as putative mitophagosome structures (red arrows). Scale bars: 200 nm. (D) ATDC5 cells stained with mitotracker and lysotracker after transfection with Usp34 siRNA or Lv-ANT1 . Scale bars: 20 μm. (E) ATDC5 cells stained with Mito-SOX after transfection with Usp34 siRNA or Lv-ANT1 . Scale bars: 20 μm.

Techniques Used: Over Expression, Western Blot, Transfection, Membrane, Staining

USP34 overexpression enhanced chondrocyte viability. (A and B) Representative images and quantitative analysis of western blot for LC3, Parkin, PINK1, and α-tubulin in ATDC5 cells transfected with Usp34 lentiviral activation particles ( Usp34 ac) following exposure to IL-1β. (C and D) Representative images and quantitative analysis of western blot for MMP13, ADAMTS5, and α-tubulin in ATDC5 cells transfected with Usp34 ac following exposure to IL-1β. (E and F) Representative images and quantitative analysis of western blot for MMP13, ADAMTS5, and α-tubulin in ATDC5 cells with the indicated treatment. (G) ATDC5 cells stained with Acan and Col2a1 after transfection with Usp34 ac or Lv-ANT1 . Scale bars: 50 μm. (H and I) Relative mRNA expression of Acan and Col2a1 in ATDC5 cells with the indicated treatments.

Figure Legend Snippet: USP34 overexpression enhanced chondrocyte viability. (A and B) Representative images and quantitative analysis of western blot for LC3, Parkin, PINK1, and α-tubulin in ATDC5 cells transfected with Usp34 lentiviral activation particles ( Usp34 ac) following exposure to IL-1β. (C and D) Representative images and quantitative analysis of western blot for MMP13, ADAMTS5, and α-tubulin in ATDC5 cells transfected with Usp34 ac following exposure to IL-1β. (E and F) Representative images and quantitative analysis of western blot for MMP13, ADAMTS5, and α-tubulin in ATDC5 cells with the indicated treatment. (G) ATDC5 cells stained with Acan and Col2a1 after transfection with Usp34 ac or Lv-ANT1 . Scale bars: 50 μm. (H and I) Relative mRNA expression of Acan and Col2a1 in ATDC5 cells with the indicated treatments.

Techniques Used: Over Expression, Western Blot, Transfection, Activation Assay, Staining, Expressing

Western Blot:

Article Title: Clinacanthus nutans Mitigates Neuronal Death and Reduces Ischemic Brain Injury: Role of NF-κB-driven IL-1β Transcription.
Article Snippet: Neuroinflammation has been shown to exacerbate ischemic brain injury, and is considered as a prime target for the development of stroke therapies.. Clinacanthus nutans Lindau (C. nutans) is widely used in traditional medicine for treating insect bites, viral infection and cancer, due largely to its anti-oxidative and anti-inflammatory properties.. Recently, we reported that an ethanol extract from the leaf of C. nutans could protect the brain against ischemia-triggered neuronal death and infarction.

Article Title: Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signalling pathway
Article Snippet: Methodology is similar to retroviral transduction, with exception of packaging vectors (pCMV-VSV-G and pCMV-dR8.91) and selected with 2 μg/ml puromycin (Thermo Fischer Scientific, #A1113803). .. For immunoblotting and co-immunoprecipitation: Actin (Santa Cruz, #sc-47778; 1:5000), FLAG-HRP (Sigma Aldrich, #A8592; 1:4000), HA-HRP (Roche, #11867423001; 1:2000), KDEL (AbCam, #ab12223; 1:2000), iRhom2-NT-specific (( ); 1:500), Myc (Abcam, #ab9132; 1:2000), SEC11A (Proteintech, #14753-1-AP; 1:500), SEC11C (Novus Biologicals, NBP1-80774; 1:500), SPCS1 (Proteintech, #11847-1-AP; 1:500), SPCS2 (Merck Life Science, #HPA013386; 1:500), SPCS3 (Santa Cruz, sc-377334; 1:500), RHBDL4 (( ); 1:1000), POL2 (MBL International, MABI0601; 1:1000), TFIID (Santa Cruz, #sc-273; 1:1000), Histone H3 (Cell Signaling, #4499T; 1:4000), GRIN2D (Novus Biologicals, NBP2-94573; 1:1000), ZNF195 (Novus Biologicals, NBP2-93054; 1:1000), DDR1 (Cell Signaling, #5583T ; 1:1000), DDX3X (Cell Signaling, #3189S; 1:1000), ADAM17 (AbCam, #ab39162; 1:2000). .. For immunofluorescence: DAPI (Thermo Fischer, #D1306; 1 μg/ml), HA (Cell Signaling Technology, #3724; 1:500), HA.11 (Enzo Life Sciences, #ABS120-0200; 1:500), BAP31 (Enzo Life Sciences, #ALX-804-601-C100; 1:250), GFP (AbCam, #ab13970; 1:500), RHBDF2 (Sigma-Aldrich, #SAB1304414), SUN2 (Atlas Antibodies, HPA001209; 1:250)

Blocking Assay:

Article Title: Sustainable Grape Antioxidant Dietary Fiber Preserves Proximal Colonic Homeostasis via Hsp27 and AMPK Signaling.
Article Snippet: Protein extracts (DC Protein Assay Kit, Bio-Rad, Madrid Spain) from each sample were separated by SDS–PAGE and transferred to polyvinylidene fluoride membranes (GE Healthcare, Madrid, Spain). .. After blocking, membranes were incubated overnight at 4 ◦C with primary antibodies against p21WAF1/Cip1 (F-5, sc-6246), p27Kip1 (F-8, sc-1641), cyclin E (HE12, sc-247), cyclin D1 (A-12, sc-8396), Bax (2D2, sc-20067), Bcl-2 (C-2, sc-7382), Bcl-xL (H-5, sc-8392), cytochrome c (A-8, sc-13156), AIF (E-1, sc-13116), Hsp70 (W27, sc-24), Hsp27 (F-4, sc-13132), AMPKα1/2 (D-6, sc-74461), p-AMPKThr172 (sc-33524), CPT1 (E-7, sc-393070), PCNA (PC10, sc-56), β-actin (C4, sc-47778; cytosolic control), TOM20 (F-10, sc-17764; mitochondrial control), and TFIID (58C9, sc-421; nuclear control) from Santa Cruz Biotechnology (anti-mouse; Quimigen, Madrid, Spain); p53 (DO-7, MA5-12557), p-p53Ser392 (44–640 G), and p-mTORSer2448 (44–1125 G) from Invitrogen (Thermo Fisher Scientific, Madrid, Spain; anti-rabbit); and p-RaptorSer792 (#2083) and p-ACCSer79 (#3661) from Cell Signaling Technology (anti-rabbit; Danvers, MA, USA). ..

Article Title: Sustainable Grape Antioxidant Dietary Fiber Preserves Proximal Colonic Homeostasis via Hsp27 and AMPK Signaling
Article Snippet: Protein extracts (DC Protein Assay Kit, Bio-Rad, Madrid Spain) from each sample were separated by SDS–PAGE and transferred to polyvinylidene fluoride membranes (GE Healthcare, Madrid, Spain). .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies against p21 WAF1/Cip1 (F-5, sc-6246), p27 Kip1 (F-8, sc-1641), cyclin E (HE12, sc-247), cyclin D1 (A-12, sc-8396), Bax (2D2, sc-20067), Bcl-2 (C-2, sc-7382), Bcl-xL (H-5, sc-8392), cytochrome c (A-8, sc-13156), AIF (E-1, sc-13116), Hsp70 (W27, sc-24), Hsp27 (F-4, sc-13132), AMPKα1/2 (D-6, sc-74461), p-AMPK Thr172 (sc-33524), CPT1 (E-7, sc-393070), PCNA (PC10, sc-56), β-actin (C4, sc-47778; cytosolic control), TOM20 (F-10, sc-17764; mitochondrial control), and TFIID (58C9, sc-421; nuclear control) from Santa Cruz Biotechnology (anti-mouse; Quimigen, Madrid, Spain); p53 (DO-7, MA5-12557), p-p53 Ser392 (44–640 G), and p-mTOR Ser2448 (44–1125 G) from Invitrogen (Thermo Fisher Scientific, Madrid, Spain; anti-rabbit); and p-Raptor Ser792 (#2083) and p-ACC Ser79 (#3661) from Cell Signaling Technology (anti-rabbit; Danvers, MA, USA). ..

Article Title: Oxidized LDL Downregulates ABCA1 Expression via MEK/ERK/LXR Pathway in INS-1 Cells
Article Snippet: .. After blocking with skim milk, the membrane was incubated with the 1st antibody for ABCA1, LXR, TFIID, Lox-1 (Santa Cruz Biotechnology Inc., Dallas, CA, USA), or phospho-MEK, MEK, phospho-ERK1/2, ERK1/2 (Cell Signal Technology, Tokyo, Japan) at 4 °C overnight or GAPDH antibody (Biomol Research, Plymouth Meeting, Pennsylvania, USA) at room temperature for 1 h [ ]. .. The membrane was then incubated with the HRP-linked rabbit or mouse secondary antibody (DakoCytomation) at room temperature for 1 h. Protein bands were detected by ECL (GE Healthcare, Tokyo, Japan) under Luminescent image analyzer LAS-1000 Plus.

Incubation:

Control:

Ubiquitin Proteomics:

Article Title: BRCA1-BARD1 regulates transcription through modulating topoisomerase IIβ
Article Snippet: The primary antibodies obtained for Bethyl Laboratories were as follows: BRCA1 (A300-000A), BARD1 (A300-263A), phosphorylated BRCA1 at S1524 (A300-001A), MED23 (A300-425A), and TOP2B (A300-949A). .. The antibodies for α -Tubulin (sc-8035), TOP2B (sc-25330), TFIID (sc-421), TFIIF (sc-37430), TFIIE α (sc-133065), CDK9 (sc-13130, and Ubiquitin (sc-8017) were from Santa Cruz Biotechnology. .. ELK1 (#91825) and Pol II (#2629) were purchased from Cell Signaling Technology.

Article Title: BRCA1-BARD1 regulates transcription through modulating topoisomerase IIβ
Article Snippet: The primary antibodies obtained from Bethyl Laboratories were as follows: BRCA1 (A300-000A), BARD1 (A300-263A), phosphorylated BRCA1 at S1524 (A300-001A), MED23 (A300-425A) and TOP2B (A300-949A). .. The antibodies for α-tubulin (sc-8035), TOP2B (sc-25330), TFIID (sc-421), TFIIF (sc-37430), TFIIE α (sc-133065), CDK9 (sc-13130) and ubiquitin (sc-8017) were from Santa Cruz Biotechnology. .. ELK1 (no. 91825) and Pol II (no. 2629) were purchased from Cell Signaling Technology.

other:

Article Title: NET-prism enables RNA polymerase-dedicated transcriptional interrogation at nucleotide resolution
Article Snippet: Antibodies used in this study: Spt6 (Cell Signalling – D6J9H); Ssrp1 (Biolegends – 10D1); TFIID (Santa Cruz – sc-273); Med14 (Invitrogen – PA5-44864), Total Pol II (anti-CTD, ab817 – Abcam), Sf1 (A303-214A; Bethyl).

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